Ma-2018-Differentially-expressed-non-coding

RESEARCHARTICLEOpenAccessDifferentiallyexpressednon-codingRNAsinducedbytransmissiblegastroenteritisviruspotentiallyregulateinflammationandNF-κBpathwayinporcineintestinalepithelialcelllineXuelianMa†,XiaominZhao†,ZhichaoZhang,JianxiongGuo,LijuanGuan,JuejunLi,MiMi,YongHuangandDewenTong*AbstractBackground:Transmissiblegastroenteritisvirus(TGEV)infectioncanactivateNF-κBpathwayinporcineintestinalepithelialcellsandresultinsevereinflammation.Non-codingRNAs(ncRNAs)arenottranslatedintoproteinsandplayanimportantroleinmanybiologicalandpathologicalprocessessuchasinflammation,viralinfection,andmitochondrialdamage.However,whetherncRNAsparticipateinTGEV-inducedinflammationinporcineintestinalepithelialcellsislargelyunknown.Results:Inthisstudy,thenext-generationsequencing(NGS)technologywasusedtoanalyzetheprofilesofmRNAs,miRNAs,andcircRNAsinMock-andTGEV-infectedintestinalporcineepithelialcell-jejunum2(IPEC-J2)cellline.Atotalof523mRNAs,65microRNAs(miRNAs),and123circularRNAs(circRNAs)weredifferentiallyexpressed.KyotoEncyclopediaofGenesandGenomes(KEGG)analysisshoweddifferentiallyexpressedmRNAswerelinkedtoinflammation-relatedpathways,includingNF-κB,Toll-likereceptor,NOD-likereceptor,Jak-STAT,TNF,andRIG-I-likereceptorpathways.TheinteractionsamongmRNA,miRNA,andcircRNAwereanalyzed.Thedatashowedthatssc_circ_009380andmiR-22mighthaveinteractionrelationship.Dual-luciferasereporterassayconfirmedthatmiR-22directlyboundtossc_circ_009380.WealsoobservedthatoverexpressionofmiR-22ledtoareductionofp-IκB-αandaccu